Petunia peroxidase a: isolation, purification and characteristics
نویسندگان
چکیده
منابع مشابه
The Isolation and Purification of Thyroid Peroxidase*
A procedure for the extraction and isolation of the peroxidase in pig thyroid glands has been described. The enzyme has been purified by gel filtration and diethylaminoethyl chromatography, and its spectral properties suggest that the enzyme is a typical hemoprotein peroxidase, while gel filtration experiments have shown it to have a molecular weight of 104,000. The specific activity of the pre...
متن کاملIsolation and Affinity Purification of Peroxidase from Daikon
Peroxidase enzyme plays an important role in pharmaceutical, Industrial and various research laboratories. This enzyme can be purified by various different method. Our research trend was to purify the enzyme in a convenient and easy way. For this purpose a new affinity column was prepare by immobilizing PABA to N-HydroxySuccinamaideagarose gel. The presence of peroxidase was investigated on var...
متن کاملIsolation and purification of rat mammary tumor peroxidase.
7,12-Dimethylbenz(a)anthracene-induced rat mammary tumors often contain high levels of the enzyme perioxidase, a putative marker of estrogen dependence. This enzyme can be effectively extracted with 0.5 M CaCl2, giving rise to a soluble peroxidase with a molecular weight of about 50,000 as determined by gel filtration. This is the same size as the estrogen-induced peroxidase of rat uterus but s...
متن کاملIsolation barriers between petunia axillaris and Petunia integrifolia (Solanaceae).
The isolation barriers restricting gene flow between populations or species are of crucial interest for understanding how biological species arise and how they are maintained. Few studies have examined the entire range of possible isolation barriers from geographic isolation to next generation hybrid viability. Here, we present a detailed analysis of isolation barriers between two flowering pla...
متن کاملStylar peroxidase and incompatibility reactions in Petunia hybrida.
Self-, cross- and non-pollinated styles of Petunia hybrida were treated with 3-3'-diaminobenzidine for the ultrastructural localization of peroxidase activity. Wall peroxidases were absent in cross-pollinated styles, but they were detectable as an osmiophilic deposit on the cell walls of the outer portion of the transmitting tissue in self- and non-pollinated styles. The cell layer showing pero...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1991
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1991.tb16101.x